Recombinant Human/Cynomolgus CD28 Protein (Biotin)
Biotinylated Recombinant Human T-cell-specific surface glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.
Product Specifications
Product Name Alternative
CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; Tp44; TP44
UniProt
P10747
Expression Region
Asn19-Pro152
Conjugation
Biotin
Tag
C-Fc-Avi
Origin
Human/Cynomolyus
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Human B7-1-His (Cat#orb2994419) on HIS1K Biosensor, can bind Biotinylated Human/Cynomolgus CD28-Fc-Avi (Cat#orb2993831) with an affinity constant of 9.09 pM as determined in BLI assay. Loaded Biotinylated Human/Cynomolgus CD28-Fc-Avi (Cat#orb2993831) on SA Biosensor, can bind Anti-Human CD28 mAb (Cat#GMP-A063) with an affinity constant of 6.4 pM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 44.1 KDa. Observed: 60-90 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mTris-HCl, 150mM NaCl, pH 8.0.
Available Sizes
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