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Recombinant Mouse IL-23 Protein

Recombinant Mouse Interleukin-23 is produced by our Baculovirus expression system and the target gene encoding Val22-Ala196&Met23-Ser335 is expressed.

Product Specifications

Product Name Alternative

SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2

UniProt

Q9EQ14, P43432

Expression Region

Val22-Ala196&Met23-Ser335

Conjugation

Unconjugated

Tag

Tag free

Origin

Mouse

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Human IL-23R-Fc (Cat#orb2994313) on Protein A Biosensor, can bind Mouse IL-23A (Cat#orb2993830) with an affinity constant of 3.81 nM as determined in BLI assay. (Regularly tested) Loaded Anti-Human-IL23-P19 mAb (Cat#orb2993116) on Pro-A Biosensor, can bind Recombinant Mouse IL-23 (Cat#orb2993830) with an affinity constant of 0.512 uM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 125.44 ng/ml.

Form

Lyophilized

Molecular Weight

Predicted: 19.7&35.8 KDa. Observed: 40&20 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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