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Recombinant Human CD3D&CD3E Heterodimer Protein

Recombinant Human CD3D&CD3E is produced by our Mammalian expression system and the target gene encoding Phe22-Ala105&Asp23-Asp126 is expressed with a human IgG1 Fc-Flag, human IgG1 Fc-6His tag at the C-terminus.

Product Specifications

Product Name Alternative

CD3E & CD3D; CD3 delta & CD3 epsilon; CD3 delta / epsilon

UniProt

P04234, P07766

Expression Region

Phe22-Ala105&Asp23-Asp126

Conjugation

Unconjugated

Tag

C-Fc-Flag&C-Fc-6His

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Anti-Human CD3E mAb-mFc (Cat#orb2992718) on AMC Biosensor, can bind Human CD3D&CD3E Heterodimer C-Fc-Flag&C-Fc-6His (Cat#orb2993825) with an affinity constant of -3 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 37&39.2 KDa. Observed: 40-60 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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