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Recombinant Human Siglec-2 Protein (Biotin)

Biotinylated Recombinant Human B-cell Receptor CD22 is produced by our Mammalian expression system and the target gene encoding Asp20-Arg687 is expressed with a 6His, Avi tag at the C-terminus.

Product Specifications

Product Name Alternative

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14

UniProt

P20273

Expression Region

Asp20-Arg687

Conjugation

Biotin

Tag

C-6His-Avi

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Biotinylated Human Siglec-2-His-Avi (Cat#orb2993816) on HIS1K Biosensor, can bind Anti-Human Siglec-2 mAb-Fc (Cat#orb2993148) with an affinity constant of 0.16 nM as determined in BLI assay. Loaded Biotinylated Human Siglec-2-His-Avi (Cat#orb2993816) on SA Biosensor, can bind Anti-Human Siglec-2 mAb-Fc (Cat#orb2993148) with an affinity constant of -3 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 78 KDa. Observed: 110-130 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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