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Recombinant Human HER2 Protein (Biotin)

Biotinylated Recombinant Human Receptor tyrosine-protein kinase ErbB-2 is produced by our Mammalian expression system and the target gene encoding Thr23-Thr652 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Product Specifications

Product Name Alternative

Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1

UniProt

P04626

Expression Region

Thr23-Thr652

Conjugation

Biotin

Tag

C-Fc-Avi

Origin

Human

Field of Research

Signal Transduction

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Anti-Human HER2 mAb (Cat#orb2993152) at 2μg/ml (100 μl/well) can bind Human HER2-Fc-Avi (Cat#orb2993812) . The ED50 of Human HER2-Fc-Avi (Cat#orb2993812) is 3.07 ng/ml.. BLI: Loaded Biotinylated Human HER2-Fc-Avi (Cat#orb2993812) on SA Biosensor, can bind Anti-Human HER2 mAb (Cat#orb2993152) with an affinity constant of -3nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 98.3 KDa. Observed: 120-140 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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