Recombinant Human TSLP Protein
Recombinant Human Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159 (R127A, R130A) is expressed with a 10His tag at the C-terminus.
Product Specifications
Product Name Alternative
Thymic stromal lymphopoietin; TSLP
UniProt
Q969D9
Expression Region
Tyr29-Gln159 (R127A, R130A)
Conjugation
Unconjugated
Tag
C-10His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Anti-Human TSLP mAb (Cat#orb2992721) at 2μg/ml (100 μl/well) can bind Human TSLP-His (Cat#orb2993808) . The ED50 of Human TSLP-His (Cat#orb2993808) is 4.48 ng/ml.. BLI: Loaded Anti-Human TSLP mAb-Fc (Cat#orb2992721) on Protein A Biosensor, can bind Human TSLP-His (Cat#orb2993808) with an affinity constant of -3 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 16.1 KDa. Observed: 20-27 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Histidine-HCl, 4% Sucrose, 4% Mannitol, 0.02% Tween 80 (w/v), pH 6.0.