Recombinant Human Nectin-4 Protein
Recombinant Human Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly32-Val351 is expressed with a mouse IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR
UniProt
Q96NY8
Expression Region
Gly32-Val351
Conjugation
Unconjugated
Tag
C-mFc
Origin
Human
Field of Research
Cancer Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human Nectin-1-His (Cat#orb2995040) at 5μg/ml (100 μl/well) can bind Human Nectin-4-mFc (Cat#orb2993807) . The ED50 of Human Nectin-4-mFc (Cat#orb2993807) is 1.7 ug/ml. (Regularly tested) . BLI: Loaded Human Nectin-4-mFc (Cat#orb2993807) on AMC Biosensor, can bind Human Nectin-1-His (Cat#orb2995040) with an affinity constant of 27.7 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 60.7 KDa. Observed: 80-90 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20 mM PB, 50 mM NaCl, 5% Sucrose, 5% Mannitol, 0.05% Tween80, pH 7.0.
Available Sizes
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