Recombinant Human Nectin-4 Protein

Recombinant Human Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly32-Val351 is expressed with a mouse IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR

UniProt

Q96NY8

Expression Region

Gly32-Val351

Conjugation

Unconjugated

Tag

C-mFc

Origin

Human

Field of Research

Cancer Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human Nectin-1-His (Cat#orb2995040) at 5μg/ml (100 μl/well) can bind Human Nectin-4-mFc (Cat#orb2993807) . The ED50 of Human Nectin-4-mFc (Cat#orb2993807) is 1.7 ug/ml. (Regularly tested) . BLI: Loaded Human Nectin-4-mFc (Cat#orb2993807) on AMC Biosensor, can bind Human Nectin-1-His (Cat#orb2995040) with an affinity constant of 27.7 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 60.7 KDa. Observed: 80-90 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.