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Recombinant Human VEGFR2 Protein (Biotin)

Biotinylated Recombinant Human Vascular Endothelial Growth Factor Receptor 2 is produced by our Mammalian expression system and the target gene encoding Ala20-Glu764 is expressed with a 6His, Avi tag at the C-terminus.

Product Specifications

Product Name Alternative

Vascular endothelial growth factor receptor 2; KDR; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1

UniProt

P35968

Expression Region

Ala20-Glu764

Conjugation

Biotin

Tag

C-6His-Avi

Origin

Human

Field of Research

Cardiovascular Research; Cell Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Biotinylated Human VEGFR2-His-Avi (Cat#orb2993796) at 1μg/ml (100 μl/well) can bind Anti-Human VEGFR2 mAb (Cat#orb2993814) .The ED50 of Anti-Human VEGFR2 mAb (Cat#orb2993814) is 44.08 ng/ml.. BLI: Loaded Anti-Human VEGFR2 mAb-Fc (Cat#orb2993814) on Protein A Biosensor, can bind Biotinylated Human VEGFR2-6His-Avi (Cat#orb2993796) with an affinity constant of 1.1 pM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 86.1 KDa. Observed: 120-150 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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