Recombinant Human Nectin-4 Protein (Biotin)
Biotinylated Recombinant Human Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly32-Val351 is expressed with a 6His, Avi tag at the C-terminus.
Product Specifications
Product Name Alternative
PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR
UniProt
Q96NY8
Expression Region
Gly32-Val351
Conjugation
Biotin
Tag
C-6His-Avi
Origin
Human
Field of Research
Cancer Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human Nectin-4-His-Avi (Cat#orb2993794) at 2μg/ml (100 μl/well) can bind Anti-Human Nectin-4 mAb-mFc (Cat#orb2993141) . The ED50 of Anti-Human Nectin-4 mAb-mFc (Cat#orb2993141) is 12.28 ng/ml. (Regularly tested) . BLI: Loaded Biotinylated Human Nectin-4-His-Avi (Cat#orb2993794) on SA Biosensor, can bind Anti-Human Nectin-4 mAb (Cat#orb2993141) with an affinity constant of 1 -3 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 37.2 KDa. Observed: 40-50 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Available Sizes
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