Recombinant Human IL-2 Protein (Biotin)

ELISA: Immobilized Human IL-2RA-His (Cat#orb2994464) at 2μg/ml (100 μl/well) can bind Human IL-2-His-Avi (Cat#orb2993791) . The ED50 of Human IL-2-His-Avi (Cat#orb2993791) is 6.45 ng/ml.. BLI: Loaded Human IL-2RA-Fc (Cat#orb2994463) on Protein A Biosensor, can bind Biotinylated Human IL-2-His-Avi (Cat#orb2993791) with an affinity constant of 8.60 nM as determined in BLI assay. Loaded Human IL-2RB-Fc (Cat#orb2994461) on Protein A Biosensor, can bind Biotinylated Human IL-2-His-Avi (Cat#orb2993791) with an affinity constant of 0.24 uM as determined in BLI assay. Loaded Mouse IL-2RA-Fc (Cat#orb2994437) on Pro A Biosensor, can bind Biotinylated Human IL-2-His-Avi (Cat#orb2993791) with an affinity constant of 15.6 nM as determined in BLI assay.. Cell Based Assay: Measured in a cell proliferation assay using CTLL‐2 mouse cytotoxic T cells. The ED50 for this effect is 0.2 ng/ml.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.