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Recombinant Human TROP-2 Protein (Biotin)

Biotinylated Recombinant Human Tumor-associated Calcium Signal Transducer 2 is produced by our Mammalian expression system and the target gene encoding His27-Thr274 is expressed with a Avi, 6His tag at the N-terminus.

Product Specifications

Product Name Alternative

Tumor-associated calcium signal transducer 2; Membrane component chromosome 1 surface marker 1; Cell surface glycoprotein Trop-2; TACSTD2; TROP2

UniProt

P09758

Expression Region

His27-Thr274

Conjugation

Biotin

Tag

C-Avi-6His

Origin

Human

Field of Research

Musculoskeletal & Connective Tissue Research

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Biotinylated Human TROP-2-Avi-His (Cat#orb2993745) at 1μg/ml (100 μl/well) can bind Anti-Human TROP-2 mAb (Cat#orb2993115) .The ED50 of Anti-Human TROP-2 mAb (Cat#orb2993115) is 4.19 ng/ml.. BLI: Loaded Anti-Human TROP-2 mAb-Fc (Cat#orb2993115) on Protein A Biosensor, can bind Biotinylated Human TROP-2-Avi-His (Cat#orb2993745) with an affinity constant of 0.49 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 30.5 KDa. Observed: 40-60 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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