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Recombinant Human BAFF Protein

Recombinant Human TNF Ligand Superfamily Member 13B is produced by our Mammalian expression system and the target gene encoding Ala134-Leu285 is expressed with a human IgG1 Fc tag at the N-terminus.

Product Specifications

Product Name Alternative

Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1

UniProt

Q9Y275

Expression Region

Ala134-Leu285

Conjugation

Unconjugated

Tag

N-Fc

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human BCMA-His-Flag (Cat#orb2993882) at 1μg/ml (100 μl/well) can bind Human BAFF-Fc (Cat#orb2994098) .The ED50 of Human BAFF-Fc (Cat#orb2994098) is 13.62 ng/ml.. BLI: Loaded Human BCMA-His (Cat#orb2993882) on HIS1K Biosensor, can bind Human BAFF-Fc (Cat#orb2994098) with an affinity constant of 0.63 nM as determined in BLI assay. Loaded Human BCMA-His (Cat#orb2994719) on HIS1K Biosensor, can bind Human BAFF-Fc (Cat#orb2994098) with an affinity constant of -3 nM as determined in BLI assay. Loaded Human BCMA-mFc (Cat#orb2994062) on AMC Biosensor, can bind Human BAFF-Fc (Cat#orb2994098) with an affinity constant of -3 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 43.3 KDa. Observed: 54 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM Tris-HCl,6% Trehalose,4% Mannitol,0.05% Tween 80,10mM Methionine, pH 8.55.

Available Sizes

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