Recombinant Human BAFF Protein
Recombinant Human TNF Ligand Superfamily Member 13B is produced by our Mammalian expression system and the target gene encoding Ala134-Leu285 is expressed with a human IgG1 Fc tag at the N-terminus.
Product Specifications
Product Name Alternative
Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1
UniProt
Q9Y275
Expression Region
Ala134-Leu285
Conjugation
Unconjugated
Tag
N-Fc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human BCMA-His-Flag (Cat#orb2993882) at 1μg/ml (100 μl/well) can bind Human BAFF-Fc (Cat#orb2994098) .The ED50 of Human BAFF-Fc (Cat#orb2994098) is 13.62 ng/ml.. BLI: Loaded Human BCMA-His (Cat#orb2993882) on HIS1K Biosensor, can bind Human BAFF-Fc (Cat#orb2994098) with an affinity constant of 0.63 nM as determined in BLI assay. Loaded Human BCMA-His (Cat#orb2994719) on HIS1K Biosensor, can bind Human BAFF-Fc (Cat#orb2994098) with an affinity constant of -3 nM as determined in BLI assay. Loaded Human BCMA-mFc (Cat#orb2994062) on AMC Biosensor, can bind Human BAFF-Fc (Cat#orb2994098) with an affinity constant of -3 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 43.3 KDa. Observed: 54 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl,6% Trehalose,4% Mannitol,0.05% Tween 80,10mM Methionine, pH 8.55.
Available Sizes
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