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Recombinant Mouse CD40L Protein

Recombinant Mouse CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.

Product Specifications

Product Name Alternative

CD40 Ligand; CD40LG; HIGM1; T-B cell-activating molecule; T-BAM; TNFSF5; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

UniProt

P27548

Expression Region

Met112-Leu260

Conjugation

Unconjugated

Tag

N-6His

Origin

Mouse

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Recombinant Mouse CD40 (C-Fc) (orb2994384) at 2μg/ml (100 μl/well) can bind Recombinant Mouse CD40L (N-6His) (orb2994091) .The EC50 of Recombinant Mouse CD40L (N-6His) (orb2994091) is 12.91ng/ml. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 18.8 KDa. Observed: 20 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.

Available Sizes

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