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Recombinant Human ROR1 Protein

Recombinant Human Inactive Tyrosine-protein Kinase Transmembrane Receptor ROR1 is produced by our Mammalian expression system and the target gene encoding Gln30-Glu403 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Inactive tyrosine-protein kinase transmembrane receptor ROR1; Neurotrophic tyrosine kinase, receptor-related 1; ROR1; NTRKR1

UniProt

Q01973

Expression Region

Gln30-Glu403

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Field of Research

Stem Cell & Developmental Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Recombinant Human ROR1 (C-6His) (Cat#orb2994090) at 2 μg/ml (100 μl/well) can bind Human ROR1 Antibody (3E8) (Cat#orb2992600) . The ED50 of Human ROR1 Antibody (3E8) (Cat#orb2992600) is 1.69 ng/ml. (Regularly tested) . BLI: Loaded Human ROR1 Antibody (3E8) -Fc (Cat#orb2992600) on Protein A Biosensor, can bind Human ROR1-His (Cat#orb2994090) with an affinity constant of 6.35 nM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human ROR1 (C-6His) (Cat#orb2994090) on HIS1K Biosensor, can bind Human ROR1 Antibody (3E8) (Cat#orb2992600) with an affinity constant of 0.163 pM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 42.8 KDa. Observed: 60-80 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM Citrate, 5% Trehalose, 1mM EDTA, 0.02% Tween80, pH 5.5.

Available Sizes

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