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Recombinant Rat PD-L1 Protein

Recombinant Rat Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Ala18-Thr238 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

UniProt

D4AE25

Expression Region

Ala18-Thr238

Conjugation

Unconjugated

Tag

C-6His

Origin

Rat

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Rat PD-L1-His (Cat#orb2994074) on HIS1K Biosensor, can bind Anti-Human PDL1 mAb-Fc (Cat#orb2993144) with an affinity constant of 0.90 nM as determined in BLI assay. (Regularly tested) Immobilized Anti-Human PDL1 mAb-Fc (Cat#orb2993144) at 8μg/ml (100 μl/well) can bind Rat PD-L1-His (Cat#orb2994074) .The ED50 of Rat PD-L1-His (Cat#orb2994074) is 0.28 ug/ml.

Form

Lyophilized

Molecular Weight

Predicted: 26.1 KDa. Observed: 40-60 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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