Recombinant Rat PD-L1 Protein
Recombinant Rat Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Ala18-Thr238 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
UniProt
D4AE25
Expression Region
Ala18-Thr238
Conjugation
Unconjugated
Tag
C-6His
Origin
Rat
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Rat PD-L1-His (Cat#orb2994074) on HIS1K Biosensor, can bind Anti-Human PDL1 mAb-Fc (Cat#orb2993144) with an affinity constant of 0.90 nM as determined in BLI assay. (Regularly tested) Immobilized Anti-Human PDL1 mAb-Fc (Cat#orb2993144) at 8μg/ml (100 μl/well) can bind Rat PD-L1-His (Cat#orb2994074) .The ED50 of Rat PD-L1-His (Cat#orb2994074) is 0.28 ug/ml.
Form
Lyophilized
Molecular Weight
Predicted: 26.1 KDa. Observed: 40-60 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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