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Recombinant Cynomolgus TIGIT Protein

Recombinant Cynomolgus T cell Immunoreceptor With Ig And ITIM Domains is produced by our Mammalian expression system and the target gene encoding Met89-Pro209 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; TIGIT; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9

UniProt

G7NXM4

Expression Region

Met89-Pro209

Conjugation

Unconjugated

Tag

C-Fc

Origin

Cynomolgus

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human Cynomolgus TIGIT-Fc (Cat#orb2994063) at 10μg/ml (100 μl/well) can bind Rhesus Macaque CD155-His (Cat#orb2994061) .The ED50 of Rhesus Macaque CD155-His (Cat#orb2994061) is 0.66 ug/ml.. BLI: Loaded Human PVR-His (Cat#orb2993514) on HIS1K Biosensor, can bind Cynomolgus TIGIT-Fc (Cat#orb2994063) with an affinity constant of 24.6 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 40.6 KDa. Observed: 45-55 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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