Recombinant Human LILRB4 Protein
Recombinant Human Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 is produced by our Mammalian expression system and the target gene encoding Gln22-Glu259 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Leukocyte immunoglobulin-like receptor subfamily B member 4; Mast cell surface glycoprotein Gp49B; CD85k; Lilrb4; Gp49b
UniProt
Q8NHJ6
Expression Region
Gln22-Glu259
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human LILRB4 (C-Fc) (Cat#orb2994032) at 2μg/ml (100 μl/well) can bind Anti-Human LILRB4 Antibody (Human IgG1) *. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Anti-Human LILRB4 Antibody (Human IgG1) is 20.20ng/ml. (Regularly tested) . BLI: Loaded Recombinant Human LILRB4 (Cat#orb2994032) on Pro-A Biosensor, can bind Recombinant Human LGALS8 (Cat#orb2995237) with an affinity constant of 0.629 uM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 53.2 KDa. Observed: 65-85 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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