Recombinant Human CD40 Protein
Recombinant Human CD40L Receptor is produced by our Mammalian expression system and the target gene encoding Glu21-Arg193 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Tumor Necrosis Factor Receptor Superfamily member 5; B-Cell Surface Antigen CD40; Bp50; CD40L Receptor; CDw40; CD40; TNFRSF5
UniProt
P25942
Expression Region
Glu21-Arg193
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human CD40L-His-Flag (Cat#orb2993075) at 10μg/ml (100 μl/well) can bind Human CD40-Fc (Cat#orb2994010) . The ED50 of Human CD40-Fc (Cat#orb2994010) is 0.45 ug/ml.. BLI: Loaded Human CD40-Fc (Cat#orb2994010) on AHC Biosensor, can bind Human CD40L-mFc (Cat#orb2993921) with an affinity constant of 0.13 nM as determined in BLI assay. (Regularly tested) Loaded Human CD40L-His (Cat#orb2994033) on HIS1K Biosensor, can bind Human CD40-Fc (Cat#orb2994010) with an affinity constant of 0.16 uM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 46.3 KDa. Observed: 50-65 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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