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Recombinant Mouse VEGF 164 Protein

Recombinant Mouse Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190 is expressed.

Product Specifications

Product Name Alternative

Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGFA164; VEGF164

UniProt

Q00731

Expression Region

Ala27-Arg190

Conjugation

Unconjugated

Tag

Tag free

Origin

Mouse

Field of Research

Cardiovascular Research; Cell Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

BLI: Loaded Mouse VEGFR2-Fc (Cat#orb2993886) on Protein A Biosensor, can bind Mouse VEGF 164 (Cat#orb2993968) with an affinity constant of 0.44 nM as determined in BLI assay. Loaded Human VEGF Antibody (5E7) -Fc (Cat#DA082) on AHC Biosensor, can bind Mouse VEGF164 (Cat#orb2993968) with an affinity constant of 3.63 nM as determined in BLI assay. Loaded Human VEGF-165 Antibody (6D9) -Fc (Cat#DA083) on AHC Biosensor, can bind Mouse VEGF164 (Cat#orb2993968) with an affinity constant of 1.07 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 19.27 KDa. Observed: 18-22 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.0.

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