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Recombinant Human /Cynomolgus CD28 Protein

Recombinant Human/Cynomolgus T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; Tp44; TP44

UniProt

P10747

Expression Region

Asn19-Pro152

Conjugation

Unconjugated

Tag

C-Fc

Origin

Human/Cynomolgus

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Anti-CD28 mAb (Cat#A063) at 2μg/ml (100 μl/well) can bind Human/Cynomolgus CD28-Fc * (Cat#orb2993961) *: Biotinylated by NHS-biotin prior to testing.The ED50 of Human/Cynomolgus CD28-Fc * (Cat#orb2993961) is 13.97 ng/ml.

Form

Lyophilized

Molecular Weight

Predicted: 42.3 KDa. Observed: 40-85 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Available Sizes

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