Recombinant Human IgG1 Fc Protein
Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330 (Asp239Glu, Leu241Met) is expressed.
Product Specifications
Product Name Alternative
G gamma-1 chain C region; IGHG1
UniProt
P01857
Expression Region
Asp104-Lys330 (Asp239Glu, Leu241Met)
Conjugation
Unconjugated
Tag
Tag free
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human IgG1 Fc (Cat#orb2993960) at 2 μg/ml (100 μl/well) can bind Recombinant Human CD64 (C-6His) (Cat#orb2994349) . The ED50 of Recombinant Human CD64 (C-6His) (Cat#orb2994349) is 30-150 ng/ml .. BLI: Loaded Human IgG1 (Cat#orb2993960) on Protein A Biosensor, can bind Human CD64-His (Cat#orb2994349) with an affinity constant of 0.11 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 25.9 KDa. Observed: 32 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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