Recombinant Human PD-1 Protein
Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Programmed cell death protein 1; hPD-1; PDCD1; CD279
UniProt
Q15116
Expression Region
Pro21-Gln167
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Cancer Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human PD-1-His (Cat#orb2993958) at 2μg/ml (100 μl/well) can bind Anti-PD1 mAb (Cat#GMP-A085) . The ED50 of Anti-PD1 mAb (Cat#GMP-A085) is 9.74 ng/ml. (Regularly tested) Immobilized Recombinant Human PD-1-His (Cat#orb2993958) at 5μg/ml (100 μl/well) can bind Human PD-L1-Fc-Avi (Cat#orb2994143) . The ED50 of Human PD-L1-Fc-Avi (Cat#orb2994143) is 0.185 ug/ml.. BLI: Loaded Anti-Human PD-1 mAb-Fc (Cat#GMP-A085) on Protein A Biosensor, can bind Human PD-1-His (Cat#orb2993958) with an affinity constant of 4.08 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 17 KDa. Observed: 30-42 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 7.5.
Available Sizes
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