Recombinant Human PD-1 Protein

ELISA: Immobilized Human PD-1-His (Cat#orb2993958) at 2μg/ml (100 μl/well) can bind Anti-PD1 mAb (Cat#GMP-A085) . The ED50 of Anti-PD1 mAb (Cat#GMP-A085) is 9.74 ng/ml. (Regularly tested) Immobilized Recombinant Human PD-1-His (Cat#orb2993958) at 5μg/ml (100 μl/well) can bind Human PD-L1-Fc-Avi (Cat#orb2994143) . The ED50 of Human PD-L1-Fc-Avi (Cat#orb2994143) is 0.185 ug/ml.. BLI: Loaded Anti-Human PD-1 mAb-Fc (Cat#GMP-A085) on Protein A Biosensor, can bind Human PD-1-His (Cat#orb2993958) with an affinity constant of 4.08 nM as determined in BLI assay.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.