Recombinant Human EGFR Isoform vIII Protein (Biotin)
Biotinylated Recombinant Human Epidermal Growth Factor Receptor/Receptor Tyrosine Protein Kinase ErbB1 is produced by our Mammalian expression system and the target gene encoding Leu25-Ser378 is expressed with a 6His, Avi tag at the C-terminus.
Product Specifications
Product Name Alternative
Epidermal growth factor receptor; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR
Expression Region
Leu25-Ser378
Conjugation
Biotin
Tag
C-6His-Avi
Origin
Human
Field of Research
Cell Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Anti-Human EGFR mAb (Cat#orb2993008) on Pro-A Biosensor, can bind Biotinylated Human EGFR vIII (C-6His-Avi) (Cat#orb2993951) with an affinity constant of 3.64 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 41.5 KDa. Observed: 60-90 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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