Recombinant Human PD-1 Protein (Biotin)
Biotinylated Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a 6His, Avi tag at the C-terminus.
Product Specifications
Product Name Alternative
Programmed cell death protein 1; hPD-1; PDCD1; CD279
UniProt
Q15116
Expression Region
Pro21-Gln167
Conjugation
Biotin
Tag
C-6His-Avi
Origin
Human
Field of Research
Cancer Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human PD-1-His-Avi (Cat#orb2993936) at 2μg/ml (100 μl/well) can bind Anti-Human PD-1 mAb (Cat#GMP-A085) . The ED50 of Human Anti-Human PD-1 mAb (Cat#GMP-A085) is 8.83 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human PD-1 mAb-Fc (Cat#GMP-A085) on Protein A Biosensor, can bind Biotinylated Human PD-1-His (Cat#orb2993936) with an affinity constant of 1.54 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 19.2 KDa. Observed: 35-45 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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