Recombinant Human IL-23A & mouse IL-12B Heterodimer Protein

ELISA: Immobilized Human IL-23R-Fc (Cat#orb2994313) at 10μg/ml (100 μl/well) can bind Human IL-23A & mouse IL-12B Heterodimer-His (Cat#orb2993931) . The ED50 of Human IL-23A & mouse IL-12B Heterodimer-His (Cat#orb2993931) is 0.48 ug/ml. Immobilized Human IL-23A & mouse IL-12B Heterodimer-His (Cat#orb2993931) at 2μg/ml (100 μl/well) can bind Anti-Human IL23-P19 mAb (Cat#orb2993116) .The ED50 of Anti-Human IL23-P19 mAb (Cat#orb2993116) is 7.47 ng/ml.. BLI: Loaded Human IL-23R-Fc (Cat#orb2994313) on Protein A Biosensor, can bind Human IL-23A & mouse IL-12B Heterodimer-His (Cat#orb2993931) with an affinity constant of 9.48 nM as determined in BLI assay. Loaded Anti-Human IL23-P19 mAb-Fc (Cat#orb2993116) on Protein A Biosensor, can bind Human IL-23A & mouse IL-12B Heterodimer-His (Cat#orb2993931) with an affinity constant of -3 nM as determined in BLI assay. . Cell Based Assay: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 357.68 ng/ml.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.