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Recombinant Human BAFF Protein

Recombinant Human TNF Ligand Superfamily Member 13B is produced by our Mammalian expression system and the target gene encoding Ala134-Leu285 is expressed with a 6His tag at the N-terminus.

Product Specifications

Product Name Alternative

Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1

UniProt

Q9Y275

Expression Region

Ala134-Leu285

Conjugation

Unconjugated

Tag

N-6His

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human BAFF-His (Cat#orb2993924) at 10μg/ml (100 μl/well) can bind Mouse BCMA-Fc (Cat#orb2994112) . The ED50 of Recombinant Mouse BCMA-Fc (Cat#orb2994112) is 0.016 ug/ml.. BLI: Loaded Human BCMA-Fc (Cat#orb2994162) on Protein A Biosensor, can bind Human BAFF-His (Cat#orb2993924) with an affinity constant of 0.02 nM as determined in BLI assay. Loaded Human BCMA-mFc (Cat#orb2994062) on AMC Biosensor, can bind Human BAFF-His (Cat#orb2993924) with an affinity constant of 0.001 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 19.3 KDa. Observed: 22 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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