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Recombinant Human 4-1BBL Protein

Recombinant Human 4-1BB Ligand is produced by our E.coli expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; TNFSF9

UniProt

P41273

Expression Region

Arg71-Glu254

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human 4-1BBL-His (Cat#orb2994593) at 10μg/ml (100 μl/well) can bind Human 4-1BB-Fc (Cat#orb2994312) .The ED50 of Human 4-1BB-Fc (Cat#orb2994312) is 16.8 ng/ml.. BLI: Loaded Human 4-1BB-Fc-His (Cat#orb2994490) on Protein A Biosensor, can bind Human 4-1BBL-His (Cat#orb2994593) with an affinity constant of 1.55 pM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 20.6 KDa. Observed: 16-20 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.4.

Available Sizes

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