Recombinant Human 4-1BBL Protein
Recombinant Human 4-1BB Ligand is produced by our E.coli expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; TNFSF9
UniProt
P41273
Expression Region
Arg71-Glu254
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human 4-1BBL-His (Cat#orb2994593) at 10μg/ml (100 μl/well) can bind Human 4-1BB-Fc (Cat#orb2994312) .The ED50 of Human 4-1BB-Fc (Cat#orb2994312) is 16.8 ng/ml.. BLI: Loaded Human 4-1BB-Fc-His (Cat#orb2994490) on Protein A Biosensor, can bind Human 4-1BBL-His (Cat#orb2994593) with an affinity constant of 1.55 pM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 20.6 KDa. Observed: 16-20 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.4.
Available Sizes
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