Recombinant Human IL-17A&17F Protein
Recombinant Human Interleukin-17A/Interleukin-17F Heterodimer is produced by our Mammalian expression system and the target gene encoding Gly24-Ala155&Arg31-Gln163 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
IL‐17A/F Heterodimer; IL-17A&IL-17F Heterodimer
UniProt
Q16552
Expression Region
Gly24-Ala155&Arg31-Gln163
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
BLI: Loaded Biotinylated Human IL-17RA -His-Avi (Cat#orb2993686) on SA Biosensor, can bind Human IL-17A&17F-His (Cat#orb2994531) with an affinity constant of 8.6 pM as determined in BLI assay. Loaded Biotinylated Human IL-17RA-Fc (Cat#orb2993676) on Pro A Biosensor, can bind Human IL-17A&17F-His (Cat#orb2994531) with an affinity constant of 10.3 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 15.1&16 KDa. Observed: 15-25 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, pH 7.4.
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