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Recombinant Human TNF RI Protein

Recombinant Human Tumor Necrosis Factor Receptor I is produced by our Mammalian expression system and the target gene encoding Leu30-Thr211 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Tumor necrosis factor receptor superfamily member 1A; TNFRSF1A; Tumor necrosis factor receptor 1; TNF-R1; TNF-RI; p55; p60; CD120a; TNFAR; TNFR1

UniProt

P19438

Expression Region

Leu30-Thr211

Conjugation

Unconjugated

Tag

C-Fc

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Recombinant Human TNF RI (C-Fc) (Cat#orb2994315) on Pro-A Biosensor, can bind Recombinant Human TNF alpha (Cat#orb3002485) with an affinity constant of 1.252 pM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RI (Cat# orb2994315) inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha (Cat#orb3002485) . The IC50 for this effect is 2.7 ng/mL. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 47.2 KDa. Observed: 60 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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