Recombinant Human TNF RI Protein
Recombinant Human Tumor Necrosis Factor Receptor I is produced by our Mammalian expression system and the target gene encoding Leu30-Thr211 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Tumor necrosis factor receptor superfamily member 1A; TNFRSF1A; Tumor necrosis factor receptor 1; TNF-R1; TNF-RI; p55; p60; CD120a; TNFAR; TNFR1
UniProt
P19438
Expression Region
Leu30-Thr211
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Recombinant Human TNF RI (C-Fc) (Cat#orb2994315) on Pro-A Biosensor, can bind Recombinant Human TNF alpha (Cat#orb3002485) with an affinity constant of 1.252 pM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RI (Cat# orb2994315) inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha (Cat#orb3002485) . The IC50 for this effect is 2.7 ng/mL. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 47.2 KDa. Observed: 60 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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