Recombinant Human TNF RII Protein

Tumor necrosis factor receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factor receptor 2; TNF-R2; TNF-RII; Tumor necrosis factor receptor type II; p75; p80 TNF-alpha receptor; CD120b

ELISA: Immobilized Recombinant Human TNF alpha (Cat#orb3002485) at 5μg/ml (100 μl/well) can bind Recombinant Human TNF RII (C-Fc) (Cat#orb2994311) . The ED50 of Recombinant Human TNF RII (C-Fc) (Cat#orb2994311) is 15.35 ng/ml. (Regularly tested) . BLI: Loaded Recombinant Human TNF RII (C-Fc) (Cat#orb2994311) on Pro-A Biosensor, can bind Recombinant Human TNF alpha (Cat#orb3002485) with an affinity constant of 25.2 pM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RII (Cat# orb2994311) inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha (Cat#orb3002485) . The IC50 for this effect is 2.5 ng/mL. (Regularly tested)

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.