Recombinant Human CTLA-4 Protein
Recombinant Human Cytotoxic T-lymphocyte Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD152; CTLA4
UniProt
P16410
Expression Region
Lys36-Asp161
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Mouse B7-2-Fc (Cat#orb2994404) at 2μg/ml (100 μl/well) can bind Human CTLA-4-His (Cat#orb2994294) .The ED50 of Recombinant Human CTLA-4-His (Cat#orb2994294) is 5-50 ng/ml. (QC verified) . BLI: Loaded Recombinant Human B7-2 (C-Fc) (Cat#orb2992753) on Pro-A Biosensor, can bind Recombinant Human CTLA-4 (C-6His) (Cat#orb2994294) with an affinity constant of 7.72 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 14.3 KDa. Observed: 20-25 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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