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Recombinant Human CTLA-4 Protein

Recombinant Human Cytotoxic T-lymphocyte Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD152; CTLA4

UniProt

P16410

Expression Region

Lys36-Asp161

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Mouse B7-2-Fc (Cat#orb2994404) at 2μg/ml (100 μl/well) can bind Human CTLA-4-His (Cat#orb2994294) .The ED50 of Recombinant Human CTLA-4-His (Cat#orb2994294) is 5-50 ng/ml. (QC verified) . BLI: Loaded Recombinant Human B7-2 (C-Fc) (Cat#orb2992753) on Pro-A Biosensor, can bind Recombinant Human CTLA-4 (C-6His) (Cat#orb2994294) with an affinity constant of 7.72 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 14.3 KDa. Observed: 20-25 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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