Recombinant Human Mesothelin Protein
Recombinant Human Mesothelin is produced by our Mammalian expression system and the target gene encoding Glu296-Ser598 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Megakaryocyte potentiating factor; mesothelin; Pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein; CAK1; MPF; MSLN; SMR; CAK1; CAK1 antigen
Expression Region
Glu296-Ser598
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human Mesothelin (C-6His) (Cat#orb2994280) at 2 μg/ml (100 μl/well) can bind Anti-Human Mesothelin mAb (Cat#orb2992719) . The ED50 of Anti-Human Mesothelin mAb (Cat#orb2992719) is 4.88 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human Mesothelin mAb-Fc (Cat#orb2992719) on Protein A Biosensor, can bind Human Mesothelin-His (Cat#orb2994280) with an affinity constant of 0.1 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 34.9 KDa. Observed: 35-50 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20 mM Hepes-NaOH, 50 mM NaCl, 8% Trehalose, 0.05% Tween 80, pH 8.0.
Available Sizes
Frequently Asked Questions
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