Recombinant Human HER2 Protein
Recombinant Human Receptor Tyrosine-protein Kinase ErbB-2 is produced by our Mammalian expression system and the target gene encoding Thr23-Thr652 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1
UniProt
P04626
Expression Region
Thr23-Thr652
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Signal Transduction
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human HER2 (Cat#orb2994263) at 1μg/ml (100 μl/well) can bind Anti-Human HER2 (Trastuzumab) (Cat#Norb2993550) .The EC50 of Anti-Human HER2 (Trastuzumab) (Cat#Norb2993550) is 3.97 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human HER2 mAb-Fc (Cat#orb2993152) on Protein A Biosensor, can bind Human HER2-His (Cat#orb2994263) with an affinity constant of 1.36 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 70.2 KDa. Observed: 90-120 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH7.4
Available Sizes
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