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Recombinant Human HER2 Protein

Recombinant Human Receptor Tyrosine-protein Kinase ErbB-2 is produced by our Mammalian expression system and the target gene encoding Thr23-Thr652 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1

UniProt

P04626

Expression Region

Thr23-Thr652

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Field of Research

Signal Transduction

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Recombinant Human HER2 (Cat#orb2994263) at 1μg/ml (100 μl/well) can bind Anti-Human HER2 (Trastuzumab) (Cat#Norb2993550) .The EC50 of Anti-Human HER2 (Trastuzumab) (Cat#Norb2993550) is 3.97 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human HER2 mAb-Fc (Cat#orb2993152) on Protein A Biosensor, can bind Human HER2-His (Cat#orb2994263) with an affinity constant of 1.36 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 70.2 KDa. Observed: 90-120 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH7.4

Available Sizes

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