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Recombinant Human CD16a Protein

Recombinant Human Fc Gamma RIIIA (F176) is produced by our Mammalian expression system and the target gene encoding Gly17-Gln208 is expressed with a 6His tag at the C-terminus. It is identical to FCGR3A158F/V in the reference.

Product Specifications

Product Name Alternative

Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3

UniProt

P08637

Expression Region

Gly17-Gln208

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Human IgG1 Fc (Cat#orb2993960) on Protein-A Biosensor, can bind Human CD16a (F176) -His (Cat#orb2994215) with an affinity constant of 1.35 uM as determined in BLI assay. (Regularly tested) Loaded Human CD16a (F176) -His (Cat#orb2994215) on HIS1K Biosensor, can bind Anti-Human HER2 mAb (Cat#GMP-A062) with an affinity constant of 3.4 uM as determined in BLI assay. Loaded Human CD16a (F176) -His (Cat#orb2994215) on HIS1K Biosensor, can bind Anti-Human CD16 mAb (Cat#GMP-A091) with an affinity constant of 0.07 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 22.7 KDa. Observed: 35-50 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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