Recombinant Human CD16a Protein
Recombinant Human Fc Gamma RIIIA (F176) is produced by our Mammalian expression system and the target gene encoding Gly17-Gln208 is expressed with a 6His tag at the C-terminus. It is identical to FCGR3A158F/V in the reference.
Product Specifications
Product Name Alternative
Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3
UniProt
P08637
Expression Region
Gly17-Gln208
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Human IgG1 Fc (Cat#orb2993960) on Protein-A Biosensor, can bind Human CD16a (F176) -His (Cat#orb2994215) with an affinity constant of 1.35 uM as determined in BLI assay. (Regularly tested) Loaded Human CD16a (F176) -His (Cat#orb2994215) on HIS1K Biosensor, can bind Anti-Human HER2 mAb (Cat#GMP-A062) with an affinity constant of 3.4 uM as determined in BLI assay. Loaded Human CD16a (F176) -His (Cat#orb2994215) on HIS1K Biosensor, can bind Anti-Human CD16 mAb (Cat#GMP-A091) with an affinity constant of 0.07 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 22.7 KDa. Observed: 35-50 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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