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Recombinant Mouse CD16 Protein

Recombinant Mouse Low Affinity IgG Fc Receptor III is produced by our Mammalian expression system and the target gene encoding Ala31-Thr215 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Low affinity immunoglobulin gamma Fc region receptor III; Fcgr3; Fc gamma Receptor III; CD-antigen 16; CD16; FcRIII; IgG Fc receptor III

UniProt

P08508

Expression Region

Ala31-Thr215

Conjugation

Unconjugated

Tag

C-6His

Origin

Mouse

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Anti-Human CD28 mAb (Cat#GMP-A063) on Protein A Biosensor, can bind Mouse CD16-His (Cat#orb2994204) with an affinity constant of 0.22 uM as determined in BLI assay. Loaded Recombinant Mouse IgG2a Fc (Cat#orb2993941) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.16 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Mouse IgG2b Fc (Cat#orb2993940) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.121 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Mouse IgG3 Fc (Cat#orb2993939) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.62 uM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 22 KDa. Observed: 35-40 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20 mM PB, 10% Sucrose, 8% Mannitol, 0.05% Tween 80, pH 7.0.

Available Sizes

Frequently Asked Questions

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