Recombinant Mouse CD16 Protein
Recombinant Mouse Low Affinity IgG Fc Receptor III is produced by our Mammalian expression system and the target gene encoding Ala31-Thr215 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Low affinity immunoglobulin gamma Fc region receptor III; Fcgr3; Fc gamma Receptor III; CD-antigen 16; CD16; FcRIII; IgG Fc receptor III
UniProt
P08508
Expression Region
Ala31-Thr215
Conjugation
Unconjugated
Tag
C-6His
Origin
Mouse
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Anti-Human CD28 mAb (Cat#GMP-A063) on Protein A Biosensor, can bind Mouse CD16-His (Cat#orb2994204) with an affinity constant of 0.22 uM as determined in BLI assay. Loaded Recombinant Mouse IgG2a Fc (Cat#orb2993941) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.16 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Mouse IgG2b Fc (Cat#orb2993940) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.121 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Mouse IgG3 Fc (Cat#orb2993939) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.62 uM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 22 KDa. Observed: 35-40 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20 mM PB, 10% Sucrose, 8% Mannitol, 0.05% Tween 80, pH 7.0.
Available Sizes
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