Recombinant Human CD32a (H167) Protein
Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor II-A (H167) is produced by our Mammalian expression system and the target gene encoding Ala36-Ile218 is expressed with a 6His tag at the C-terminus. It is identical to FCGR2A131H/R in the reference.
Product Specifications
Product Name Alternative
Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32; FCGR2A; FCG2; FCGR2A1; IGFR2
UniProt
P12318
Expression Region
Ala36-Ile218
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Human IgG1 Fc (Cat#orb2993960) on Protein-A Biosensor, can bind Human CD32a-His (H167) (Cat#orb2994198) with an affinity constant of 0.666 uM as determined in BLI assay. (Regularly tested) Loaded Human CD32a-His (Cat#orb2994198) on HIS1K Biosensor, can bind Anti-Human HER2 mAb (Cat#GMP-A062) with an affinity constant of 0.6 uM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 21.1 KDa. Observed: 25-32 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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