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Recombinant Human CD32a (H167) Protein

Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor II-A (H167) is produced by our Mammalian expression system and the target gene encoding Ala36-Ile218 is expressed with a 6His tag at the C-terminus. It is identical to FCGR2A131H/R in the reference.

Product Specifications

Product Name Alternative

Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32; FCGR2A; FCG2; FCGR2A1; IGFR2

UniProt

P12318

Expression Region

Ala36-Ile218

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Human IgG1 Fc (Cat#orb2993960) on Protein-A Biosensor, can bind Human CD32a-His (H167) (Cat#orb2994198) with an affinity constant of 0.666 uM as determined in BLI assay. (Regularly tested) Loaded Human CD32a-His (Cat#orb2994198) on HIS1K Biosensor, can bind Anti-Human HER2 mAb (Cat#GMP-A062) with an affinity constant of 0.6 uM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 21.1 KDa. Observed: 25-32 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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