Recombinant Human IL-4 RA Protein
Recombinant Human Interleukin-4 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Met26-Gln231 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; IL-4-binding protein; IL4-BP; IL4R; IL4RA
UniProt
P24394
Expression Region
Met26-Gln231
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human IL-4 RA-Fc (Cat#orb2994196) at 5μg/ml (100 μl/well) can bind Human IL-4 * (Cat#orb3002477) .*: Biotinylated by NHS-biotin prior to testing.The ED50 of Human IL-4 (Cat#orb3002477) is 2.43 ng/ml.. BLI: Loaded Human IL-4-His (Cat#orb3002435) on HIS1K Biosensor, can bind Human IL-4RA-Fc (Cat#orb2994196) with an affinity constant of 0.12 nM as determined in BLI assay. . Cell Based Assay: Measured by its ability to inhibit IL-4-dependent proliferation of TF‐1 human erythroleukemic cells.The ED50 for this effect is 5-20 ng/ml. (QC verified)
Form
Lyophilized
Molecular Weight
Predicted: 50.2 KDa. Observed: 60-70 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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