Recombinant Human PD-L1 Protein (Biotin)
Biotinylated Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.
Product Specifications
Product Name Alternative
Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1
UniProt
Q9NZQ7
Expression Region
Phe19-Thr239
Conjugation
Biotin
Tag
C-Fc-Avi
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human PD-1-His (Cat#orb2993958) at 5μg/ml (100 μl/well) can bind Human PD-L1-Fc-Avi (Cat#orb2994143) . The ED50 of Human PD-L1-Fc-Avi (Cat#orb2994143) is 0.185 ug/ml.. BLI: Loaded Mouse PD-1-His (Cat#orb2994450) on HIS1K Biosensor, can bind Biotinylated Human PD-L1-Fc-Avi (Cat#orb2994143) with an affinity constant of -3 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 54.2 KDa. Observed: 70-95 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Available Sizes
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