Recombinant Human LAG-3 Protein
Recombinant Human Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Leu23-Leu450 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Lymphocyte activation gene 3 protein; LAG3; LAG-3; Protein FDC; CD223
UniProt
P18627
Expression Region
Leu23-Leu450
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human LAG-3-His (Cat#orb2994123) at 5μg/ml (100 μl/well) can bind Human FGL1-mFc (Cat#orb2994023) .The ED50 of Recombinant Human FGL1-mFc (Cat#orb2994023) is 30-90 ng/ml.. BLI: Loaded Human FGL1-Fc (Cat#orb2993935) on Protein A Biosensor, can bind Human LAG-3-His (Cat#orb2994123) with an affinity constant of 88.12nM as determined in BLI assay. Loaded Anti-Human LAG-3 mAb-Fc (Cat#Norb3002363) on Protein A Biosensor, can bind Human LAG-3-6His (Cat#orb2994123) with an affinity constant of 0.41 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 47.2 KDa. Observed: 60-80 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, 2mM EDTA, pH 7.4.
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