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Recombinant Human LAG-3 Protein

Recombinant Human Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Leu23-Leu450 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Lymphocyte activation gene 3 protein; LAG3; LAG-3; Protein FDC; CD223

UniProt

P18627

Expression Region

Leu23-Leu450

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human LAG-3-His (Cat#orb2994123) at 5μg/ml (100 μl/well) can bind Human FGL1-mFc (Cat#orb2994023) .The ED50 of Recombinant Human FGL1-mFc (Cat#orb2994023) is 30-90 ng/ml.. BLI: Loaded Human FGL1-Fc (Cat#orb2993935) on Protein A Biosensor, can bind Human LAG-3-His (Cat#orb2994123) with an affinity constant of 88.12nM as determined in BLI assay. Loaded Anti-Human LAG-3 mAb-Fc (Cat#Norb3002363) on Protein A Biosensor, can bind Human LAG-3-6His (Cat#orb2994123) with an affinity constant of 0.41 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 47.2 KDa. Observed: 60-80 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, 2mM EDTA, pH 7.4.

Available Sizes

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