Recombinant Human LAG-3 Protein
Recombinant Human Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Leu23-Leu450 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Lymphocyte activation gene 3 protein; LAG3; LAG-3; Protein FDC; CD223
UniProt
P18627
Expression Region
Leu23-Leu450
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human LAG-3-His (Cat#orb2994123) at 5μg/ml (100 μl/well) can bind Human FGL1-mFc (Cat#orb2994023) .The ED50 of Recombinant Human FGL1-mFc (Cat#orb2994023) is 30-90 ng/ml.. BLI: Loaded Human FGL1-Fc (Cat#orb2993935) on Protein A Biosensor, can bind Human LAG-3-His (Cat#orb2994123) with an affinity constant of 88.12nM as determined in BLI assay. Loaded Anti-Human LAG-3 mAb-Fc (Cat#Norb3002363) on Protein A Biosensor, can bind Human LAG-3-6His (Cat#orb2994123) with an affinity constant of 0.41 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 47.2 KDa. Observed: 60-80 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, 2mM EDTA, pH 7.4.
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog